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bio rad dc protein assay kit ii  (Bio-Rad)


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    Structured Review

    Bio-Rad bio rad dc protein assay kit ii
    Bio Rad Dc Protein Assay Kit Ii, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+dc+protein+assay+kit/DC+Protein+Assay+Kit+II/pmc13141651-193-6-6
    Average 96 stars, based on 1633 article reviews
    bio rad dc protein assay kit ii - by Bioz Stars, 2026-09
    96/100 stars

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    Protein Concentration:

    Article Title: Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genome
    Article Snippet: .. Protein concentration was determined using Bio-Rad DC protein assay kit. .. Lysates were pre-cleared with 25 μl DynabeadsTM Protein G magnetic beads (Invitrogen #10003D) for 1 h at 4°C.


    Article Title: Integrated analysis of insulin resistance reveals metabolic remodeling following diet switch–triggered calorie reduction
    Article Snippet: .. Supernatant was collected, and protein concentration was quantified using Bio-Rad DC Protein Assay Kit (5000113, 5000114, and 5000115). .. Protein lysates were mixed with 4× NuPAGE LDS buffer (Thermo Fisher Scientific, NP0007) containing dithiothreitol (final concentration of 50 mM) and denatured for 10 min at 65°C (note: samples were not heated for ADRB3 immunoblotting).

    DC Protein Assay:

    Article Title: Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genome
    Article Snippet: .. Protein concentration was determined using Bio-Rad DC protein assay kit. .. Lysates were pre-cleared with 25 μl DynabeadsTM Protein G magnetic beads (Invitrogen #10003D) for 1 h at 4°C.

    Article Title: Cytotoxic and apoptotic effects of kaempferol 3-O-rhamnoside from Schima wallichii in HepG2 cells.
    Article Snippet: For cytotoxicity and apoptosis assays, reagents included DMSO, CCK-8 (Dojindo, Japan), camptothecin (Merck, Germany), Annexin V-FLUOS Kit (BD Biosciences, USA). .. Protein quantification was performed using the Bio-Rad DC Protein Assay Kit. .. Major instruments included a rotary evaporator (Buchi R-300), microplate reader (BioTek, USA), fluorescence spectrophotometer (Twinkle LB970, Berthold, Germany), and flow cytometers (FACSCalibur and FACScan, BD, USA).


    Article Title: MDM2 Drives Proteasome Inhibitor Resistance and Represents a TP53-Independent Therapeutic Vulnerability in Multiple Myeloma.
    Article Snippet: Protein extracts were prepared using Lysis Buffer containing 20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1 mM PMSF, 10 mM NaF, 1 mM Na3VO4, and Protease Inhibitor Cocktail (Roche). .. Total protein concentrations were measured using the Bio-Rad DC protein assay kit (Bio-Rad, Hercules, CA, USA). ..

    Article Title: HDAC8 impairs tight junctions in allergic rhinitis through Smad7 deacetylation
    Article Snippet: .. Protein concentrations were determined using the Bio-Rad DC Protein Assay Kit. .. For each IP reaction, 500 μg of total protein was pre-cleared by incubation with 20 μL of Protein A/G agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA) for 1 hour at 4 °C with gentle rotation.

    Article Title: Integrated analysis of insulin resistance reveals metabolic remodeling following diet switch–triggered calorie reduction
    Article Snippet: .. Supernatant was collected, and protein concentration was quantified using Bio-Rad DC Protein Assay Kit (5000113, 5000114, and 5000115). .. Protein lysates were mixed with 4× NuPAGE LDS buffer (Thermo Fisher Scientific, NP0007) containing dithiothreitol (final concentration of 50 mM) and denatured for 10 min at 65°C (note: samples were not heated for ADRB3 immunoblotting).

    Article Title: Audiogenic Kindling Stimulation Fails to Induce Cerebral Overexpression of P-Glycoprotein and Limbic Crises in the GASH/Sal Model of Epilepsy
    Article Snippet: Protein extraction from the inferior colliculus and hippocampus of naïve and AUKstimulated GASH/Sal animals was performed using radioimmunoprecipitation assay buffer (RIPA, Cell Signaling Technology, Danvers, MA, USA, Cat. No. 9806S) supplemented with a protease inhibitor cocktail (Thermo Fisher Scientific Inc., Waltham, MA USA, Cat. No. 78442). .. Tissue homogenates were centrifuged at 14,000× g for 15 min, and protein concentrations of the resulting supernatant were determined using the Bio-Rad DC Protein Assay Kit (Bio-Rad, Hercules, CA, USA, Cat. No. 500-0116). https://doi.org/10.3390/ijms27083377 Abbreviations of amplified genes: Abcb1a: ATP binding cassette, subfamily B, member 1, isoform a; Abcb1b: ATP binding cassette, subfamily B, member 1, isoform b; β-act: beta actin; COX-2: cyclooxygenase 2; EPO-R: erythropoietin receptor; Gabr-b1: beta 1 subunit of the GABA-A receptor; H1f-1 α: hypoxia-inducible factor 1 alpha subunit; MMP-3: metalloproteinase 3; Ntrk2: neurotrophin tyrosine kinase receptor 2; Slc17a6: vesicular glutamate transporter. .. Protein samples were denatured by heating at 95 ◦C for 5 min in the presence of 10× NuPAGETM Sample Reducing Agent (Thermo Fisher Scientific Inc., Cat. No. NP0004,) and 4× NuPAGETM LDS Sample Buffer (Thermo Fisher Scientific Inc., Cat. No. NP0007).

    Article Title: Glutamate Carboxypeptidase II (GCPII)-Targeted PET to Identify Muscle Denervation in Peripheral Nervous System Injuries
    Article Snippet: .. Finally, the total protein was quantified per the manufacturer’s instructions using the Bio-Rad DC Protein Assay kit (Cat# 5000111), and GCPII activity data were calculated as femtomoles of NAAG hydrolysis per mg protein per hour (fmol/mg/h). ..

    Amplification:

    Article Title: Audiogenic Kindling Stimulation Fails to Induce Cerebral Overexpression of P-Glycoprotein and Limbic Crises in the GASH/Sal Model of Epilepsy
    Article Snippet: Protein extraction from the inferior colliculus and hippocampus of naïve and AUKstimulated GASH/Sal animals was performed using radioimmunoprecipitation assay buffer (RIPA, Cell Signaling Technology, Danvers, MA, USA, Cat. No. 9806S) supplemented with a protease inhibitor cocktail (Thermo Fisher Scientific Inc., Waltham, MA USA, Cat. No. 78442). .. Tissue homogenates were centrifuged at 14,000× g for 15 min, and protein concentrations of the resulting supernatant were determined using the Bio-Rad DC Protein Assay Kit (Bio-Rad, Hercules, CA, USA, Cat. No. 500-0116). https://doi.org/10.3390/ijms27083377 Abbreviations of amplified genes: Abcb1a: ATP binding cassette, subfamily B, member 1, isoform a; Abcb1b: ATP binding cassette, subfamily B, member 1, isoform b; β-act: beta actin; COX-2: cyclooxygenase 2; EPO-R: erythropoietin receptor; Gabr-b1: beta 1 subunit of the GABA-A receptor; H1f-1 α: hypoxia-inducible factor 1 alpha subunit; MMP-3: metalloproteinase 3; Ntrk2: neurotrophin tyrosine kinase receptor 2; Slc17a6: vesicular glutamate transporter. .. Protein samples were denatured by heating at 95 ◦C for 5 min in the presence of 10× NuPAGETM Sample Reducing Agent (Thermo Fisher Scientific Inc., Cat. No. NP0004,) and 4× NuPAGETM LDS Sample Buffer (Thermo Fisher Scientific Inc., Cat. No. NP0007).

    Binding Assay:

    Article Title: Audiogenic Kindling Stimulation Fails to Induce Cerebral Overexpression of P-Glycoprotein and Limbic Crises in the GASH/Sal Model of Epilepsy
    Article Snippet: Protein extraction from the inferior colliculus and hippocampus of naïve and AUKstimulated GASH/Sal animals was performed using radioimmunoprecipitation assay buffer (RIPA, Cell Signaling Technology, Danvers, MA, USA, Cat. No. 9806S) supplemented with a protease inhibitor cocktail (Thermo Fisher Scientific Inc., Waltham, MA USA, Cat. No. 78442). .. Tissue homogenates were centrifuged at 14,000× g for 15 min, and protein concentrations of the resulting supernatant were determined using the Bio-Rad DC Protein Assay Kit (Bio-Rad, Hercules, CA, USA, Cat. No. 500-0116). https://doi.org/10.3390/ijms27083377 Abbreviations of amplified genes: Abcb1a: ATP binding cassette, subfamily B, member 1, isoform a; Abcb1b: ATP binding cassette, subfamily B, member 1, isoform b; β-act: beta actin; COX-2: cyclooxygenase 2; EPO-R: erythropoietin receptor; Gabr-b1: beta 1 subunit of the GABA-A receptor; H1f-1 α: hypoxia-inducible factor 1 alpha subunit; MMP-3: metalloproteinase 3; Ntrk2: neurotrophin tyrosine kinase receptor 2; Slc17a6: vesicular glutamate transporter. .. Protein samples were denatured by heating at 95 ◦C for 5 min in the presence of 10× NuPAGETM Sample Reducing Agent (Thermo Fisher Scientific Inc., Cat. No. NP0004,) and 4× NuPAGETM LDS Sample Buffer (Thermo Fisher Scientific Inc., Cat. No. NP0007).

    Activity Assay:

    Article Title: Glutamate Carboxypeptidase II (GCPII)-Targeted PET to Identify Muscle Denervation in Peripheral Nervous System Injuries
    Article Snippet: .. Finally, the total protein was quantified per the manufacturer’s instructions using the Bio-Rad DC Protein Assay kit (Cat# 5000111), and GCPII activity data were calculated as femtomoles of NAAG hydrolysis per mg protein per hour (fmol/mg/h). ..



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